News

02.03.2011

Lithium Assay - new enzymatic test

Diazyme’s enzymatic lithium assay provides a convenient, stable, non-caustic low cost alternative to expensive ISE and highly caustic alkaline lithium methods. Diazyme’s enzymatic lithium assay demonstrates an excellent correlation with ISE methods and offers excellent precision (%CV <5%) and demonstrates no significant interference from all tested endogenous ions as well as ascorbic acid (5.0 mM), triglycerides (250 mg/dL), and bilirubin (45 mg/dL).

Assay Principle
The Diazyme enzymatic Lithium assay is based on a lithium sensitive enzyme whose activity is lithium concentration dependent. The enzyme, a phosphatase, converts its substrate adenosine biphosphate (PAP) to hypoxanthine through a coupled enzymatic reaction to generate uric acid and hydrogen peroxide (H2O2). H2O2 generated is then quantified by a Trinder reaction.