A simple and high throughput microplate method for extracting histone proteins used for a variety of applications, which include histone modifications such as acetylation, methylation, and sumoylation. 192 extractions.
This kit is also specifically designed to meet the requirements of histone extracts used in EpiQuik histone quantification assays. The kit can be used to directly extract histones from mammalian cells cultured in a 96-well plate. The EpiQuik Total Histone Extraction HT Kit has the fastest procedure available on the market, allowing completion within 60 minutes.
• Pre-optimized and simple 1 hour protocol.
• Conveniently includes all essential reagents to carry out a histone extraction.
• Standardized procedure for reproducible results.
• Extracts a high yield of total core histones from as little as 104 cells.
• Post-translational modifications are kept intact.
• Does not affect histone modification status or levels.