A complete set of optimized buffers and reagents for extracting total core histone proteins (H2A, H2B, H3, and H4) from mammalian cells or tissues in a simple 60 minute procedure. 100 extractions.
The post-translational modifications (PTM) in the histone extracts are kept intact and thus can be used with Epigentek's histone modification assay kits or in a variety of downstream applications for histone methylation, acetylation, phosphorylation, sumoylation, ubiquitination, citrullination, and ADP-ribosylation studies.
• Pre-optimized and simple 1 hour protocol.
• Conveniently includes all essential reagents to carry out a histone extraction.
• Standardized procedure for reproducible results.
• Extracts a high yield of total core histones from as little as 1 mg of tissues.
• Post-translational modifications are kept intact.
• Does not affect histone modification status or levels.