A complete set of essential components which enables the experimenter to quantify levels of histone H3 proteins independent of their modified states, and it can also be used for normalizing the modified histone H3 content of samples when run in parallel with Epigentek histone modification quantification kit series.
The EpiQuik Total Histone H3 Quantification Kit (Colorimetric) is suitable for specifically measuring total histone H3 using human, mouse, and rat samples, including fresh and frozen tissues and cultured adherent and suspension cells.
• Quick and efficient procedure, which can be finished within 2.5 hours.
• Innovative colorimetric assay without need for radioactivity, electrophoresis, and chromatography.
• Specifically captures histone H3 with the detection limit as low as 10 ng/well and detection range from 20 ng - 1 ug/well of histone extracts.
• The unmodified histone H3 control is conveniently included for the quantification of the amount of total histone H3.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.